摘要
运用单细胞凝胶电泳技术,研究了太原市大气细颗粒物(PM2.5)对分离的大鼠肺泡巨噬细胞 DNA 的损伤作用.结果表明,在 PM2.5浓度分别为 0,75,150,300,450μg/mL 的染毒条件下,染毒 1h 后,大鼠肺泡巨噬细胞 DNA 拖尾长度分别为 2.82,6.76,10.25,14.47,23.87μm;染毒 4h后,DNA 拖尾长度分别为 2.80,18.15,32.90,43.22,55.51μm;染毒 10h 后,DNA 拖尾长度分别为 2.49,26.57,39.73,52.10,70.09μm.由此可见,PM2.5可引起大鼠肺泡巨噬细胞 DNA 的损伤,且随着 PM2.5浓度的增加及染毒时间的延长而加剧,呈明确的剂量-效应关系和时间-效应关系.
Damage from atmospheric fine particles (PM2.5) in isolated DNA of alveolar macrophages of rats in Taiyuan City was studied applying single cell gel electrophoresis technique. Under the toxic condition of PM2.5 with concentrations of 0, 75, 150, 300, 450 μg/mL respectively, after toxicosis of 1 h, tail lengths of nuclear DNA in the alveolar macrophages from rats were 2.82, 6.76, 10.25, 14.47, 23.87 μm respectively; after toxicosis of 4 h, the tail lengths were 2.80, 18.15, 32.90, 43.22, 55.51 μm respectively; after toxicosis of 10 h, the tail lengths were 2.49, 26.57, 39.73, 52.10, 70.09 μm respectively. This showed that PM2.5 could induce the DNA damage in alveolar and aggravate with increase of particle concentration and prolongation of toxicosis time, emerging dearly the dosage-effect and time-effect relations.
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2005年第1期15-17,共3页
China Environmental Science
基金
国家自然科学基金资助项目(30230310)
