摘要
目的 研究补肾活血中药对体外培养成骨细胞活性的影响。方法 取二次酶消化法培养的胎鼠颅骨第三代成骨细胞,随机分为 5 组,即:细胞对照组、空白血清组、含药血清组、原药组、阳性对照药αD3 组,分别加入普通细胞培养液、含空白血清及不同浓度补肾活血中药含药血清、补肾活血中药原药液、阳性对照药(αD3)的条件培养液,分别在培养第 3 天和第 6 天进行各试验组成骨细胞碱性磷酸酶及骨钙蛋白分泌量测定,观察补肾活血中药含药血清及原药液对成骨细胞活性的影响。结果 含药血清组成骨细胞活性指标碱性磷酸酶(ALP)值在培养第3 天均低于空白血清组,且与含药血清浓度呈负相关关系;第 6 天,含药血清组各浓度组 ALP 值明显升高,均显著高于空白血清组,ALP 值与含药血清浓度存在正相关关系;空白血清组 ALP 值在培养第 6 天时下降,其均值低于培养第 3 天 ALP 值。培养第 3 天及第 6 天,各浓度原药组 ALP 值均高于细胞对照组及αD3 组;ALP 值与原药浓度呈正相关关系。含药血清组及中药原药组骨钙素(OC)含量显著高于空白血清及其他各对照组。结论 补肾活血中药含药血清及原药能够直接作用于体外培养的成骨细胞,促进成骨细胞增殖,增强其分泌活性;可延长成骨细胞活性分泌期,使其活性分泌高峰后移而峰值升高。
Objective To explicate the effects of tonify kidney and activate blood Chinese medicine on rat osteoblasts in vitro, and to investigate the mechanism how the Chinese medicine promotes bone formation. Methods The osteoblasts were isolated from newbone rat calvaria by sequential enzyme digestion, and the third cells which were divided into five groups randomly were treated with different concentrations of the condition culture medium. After 3 and 6 days of incubation, the effects on alkaline phosphatase (ALP) and osteocalcin (OC) secretion of tonify kidney and activate blood Chinese medicine on proliferation of the osteoblasts were observed with biochemistry and radioimmunity assay. Results The tonify kidney and activate blood Chinese medicine could enhance the secretory activty of the osteoblasts in dose dependent manner. Conlusion The tonofy-kidney and activeate blood Chinese medicine could enhance the secretory activty and prolong the secretion phase of the osteoblasts in dose-dependent manner.
出处
《中国中医药信息杂志》
CAS
CSCD
2005年第1期40-42,共3页
Chinese Journal of Information on Traditional Chinese Medicine
