摘要
目的 :制备壳聚糖口腔复合膜 ,并通过建立口腔溃疡的动物模型 ,观察复合药膜对实验性口腔溃疡愈合的影响。方法 :选择壳聚糖作为膜载体 ,加入芦荟、表皮生长因子 (EGF)等药物制备口腔复合膜 ,用动物实验验证复合膜的疗效。结果 :(1)复合膜厚约 0 .5mm ,复合膜每平方厘米含水溶性壳聚糖 0 .0 0 7g、芦荟全叶冻干粉 0 .0 5 1g、EGF 40IU、丁卡因 0 .5 3 3mg。(2 )不同时间复合膜组溃疡面积均小于对照组 (P <0 .0 5 ) ;平均愈合速度复合膜组快于其他组 (P <0 .0 5 ) ;水肿充血程度明显好于对照组 ;炎性细胞在各个时间点复合膜组均少于对照组 (P <0 .0 5 ) ;成纤维细胞高于对照组 (P <0 .0 5 )。结论 :壳聚糖复合药膜可明显促进实验性口腔溃疡愈合 ,具有一定的应用前景 ,但作用机制有待进一步阐明。
Objective: To develop a type of oral biofilm to treat ora l ulcer.Methods:Chitosan film was prepared with chitosan only,comp osite chitosan film was prepared with chitosan(7 mg/cm 2),aloe (51 mg/cm 2),di caine(0.55 mg/cm 2) and EGF(40 IU/cm 2).Oral ulcer was made by local applicati on of crystal NaOH in 90 SD rats. The rats were divided into 3 groups with 30 in each. The oral ulcer in each group was treated by chitosan film(group C), compo site chitosan film(group CC) or without treatment(control). The ulcer healing wa s obsered by naked eye and examined pathologically. Results:The average healing time(d) of the ulcer in the groups of C,CC and control was 6.2 5 ± 1.29 ,5.58±1.3 and 8.75±2.49 respectively (C or CC vs Control P<0. 05,C vs CC P>0.05). 2 days after treatment higher PCNA expression was observ ed in group CC than that in control(P<0.05), 4 and 6 days after treatment hi gher PCNA expression was observed in group C and CC than that in control(P<0 .05), more newly formed fibroblasts and blood vessles were found in group C and CC than those in control 4 days after treatment(P<0.05).Conclusion: Composite film of chitosan, aloe, dicaine and EGF may accelerate the hea ling of oral ulcer.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2005年第1期102-105,共4页
Journal of Practical Stomatology
基金
昆明医学院中青年学科带头人基金资助课题 (2 0 0 2 0 1 9)
