摘要
目的研究肝细胞癌(hepatocellular carcinoma, HCC)经导管动脉化疗栓塞(transcatheter arterial chemoembolization, TACE)后残癌组织微血管密度(microvessel density, MVD)、微血管直径、血管内皮细胞生长因子(vascular endothelial growth factor, VEGF)的表达情况及其意义.方法经病理证实的HCC 63例,包括单纯手术切除42例(对照组),TACE术后行Ⅱ期手术切除21例(TACE组).TACE组患者手术前接受1～2次不等的TACE治疗,均按统一规范标准给予化疗药物灌注+栓塞治疗.对手术切除标本进行免疫组织化学染色,其中TACE组取病灶边缘残存肿瘤部分,检测肿瘤组织的MVD、微血管直径及肿瘤细胞VEGF的表达.结果对照组MVD值为(51.69±18.17)条,TACE组MVD值为(58.57±15.75)条,二者之间比较差异无统计学意义 (t=1.48, P>0.05);对照组微血管直径为 (17.62±10.54)μm,TACE组微血管直径为 (15.79±7.65)μm,二者之间比较差异无显著性意义 (t=0.71, P>0.05).对照组VEGF阳性染色细胞数为138.26±65.24,TACE组VEGF阳性染色细胞数为243.66±88.88,二者之间比较差异有统计学意义 (t=5.34, P<0.01),TACE显著上调了VEGF蛋白的表达;VEGF阳性染色细胞数与MVD明显相关(r=0.4936, t=4.4329, P<0.05).结论化疗栓塞术后残癌组织存在丰富的肿瘤血供,残癌组织肿瘤细胞VEGF表达明显升高,新生血管生成及VEGF的表达可能在栓塞后残癌组织血供重建中起到重要的作用.
Objective To investigate the significance of intratumoral microvessel density (MVD), microvessel caliber, and expression of vascular endothelial growth factor (VEGF) in survival hepatocellular carcinoma (HCC) tissues after transcatheter arterial chemoembolization (TACE). Methods Sixty-three cases of HCC specimens verified by histopathology were studied, which included 42 cases treated with surgical resection alone (control group) and 21 cases undergoing 1-2 times TACE therapy prior toⅡ-phase surgical resection (TACE group). There was no significant difference in clinical features between the two groups. The MVD, diameter of microvessel, and VEGF protein expression in HCC tissue were measured in the two groups, respectively. Results The MVD was 51.69±18.17 in the control group and 58.57±15.75 in the TACE group. There was no significant difference between the two groups (t=1.48, P>0.05). The diameter of microvessel was (17.62±10.54)μm in the control group and (15.79±7.65)μm in the TACE group. There was no significant difference between the two groups (t=0.71, P>0.05). The cell number of VEGF expression was 243.66±88.88 in the TACE group, higher than that in the control group (138.26±65.24, t=5.34, P<0.01). There was a positive correlation between the expression of VEGF and MVD (r=0.4936, t=4.4329, P<0.05). Conclusion The study indicates that the survival cancerous tissue has rich vascularity, and expression of VEGF of the cancerous cells can be enhanced by TACE, which may play an important role in re-establishment of blood supply to tumor after TACE.
出处
《中华放射学杂志》
CAS
CSCD
北大核心
2005年第2期204-206,共3页
Chinese Journal of Radiology
基金
山东省教育厅资助课题(J99K58)
山东省科学技术委员会科技攻关项目(1998BB1CCA1)
关键词
TACE
VEGF
MVD
表达
对照组
微血管
癌组织
边缘
规范标准
显著性
Carcinoma, hepatocellular
Chemoembolization, therapeutic
Neovascularization, pathologic
Endothelial growth factors
