摘要
目的 :用一种接近临床的骨髓移植模型 ,观察骨髓基质干细胞 (MSCs)移植到缺血心肌后是否在心肌的微环境中可以向心肌细胞分化 ,提高心功能。方法 :采用自体MSCs移植的方法 ,MSCs在体外培养扩增。在通过结扎冠状动脉造成心肌缺血 1周后 ,MSCs被 5溴 - 2脱氧尿苷 (BrdU)标记后移植到自体的缺血心肌中。结果 :移植4周后 ,MSCs向心肌细胞分化 ,表达出α-横纹肌肌动蛋白 (sarcomericactin) ,缺血区血管密度明显增加 ,左室收缩功能明显强于对照组。结论 :MSCs移植可以提高缺血心肌的心功能可能和MSCs向心肌细胞分化有关。
AIM: We tested the hypothesis that marrow s tr omal cells (MSCs), when implanted into self-myocardium, can undergo milieu-depen dent differentiation, express cardiomyogenic phenotypes and enhance angiogenesis and cardiac function of ischemic hearts in vivo. METHODS: In order to achieve a safe and persistent effect, we ex plored the potential of autologous MSCs transplantation. One week after myo cardial infarction induced by occlusion of left anterior descending artery, auto logous MSCs labeled with BrdU (bromodeoxyuridine) in vitro was administered intramyocardially into the infarct area of the same donor rabbits. RESULTS: By 1 months, transplanted MSCs demonstrated to be myoge nic differentiation with the expression of α-sarcomeric actin (5C5). MSCs impla ntation significantly increased vascular density in the infarct zone and resulte d in markedly improved the left ventricular contractility. CONCLUSION: The finding indicates that autologous MSCs transplan tation may represent a promising therapeutic strategy with free of ethical conce rns and immune rejection.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2005年第2期356-360,共5页
Chinese Journal of Pathophysiology
关键词
干细胞
心肌梗死
兔
Stem cells
Myocardial infarction
Rabbits
