摘要
目的:探讨大黄对大鼠胃排空运动的影响及其机制,为临床应用大黄提供理论依据。方法:32只Wistar大鼠随机分为大黄组及对照组,分别给大鼠灌服大黄水提液或蒸馏水1h、6h后,以葡聚糖蓝-2000为胃肠内标记物观察大鼠胃排空的变化,同时用免疫组化染色观察胃窦肌间神经丛P物质(SP)、血管活性肠肽(VIP)的分布变化。结果:灌服大黄水提液后,大鼠胃排空运动显著抑制(P<0.05或P<0.01),胃窦肌间神经丛SP的分布明显减少(P<0.05或P<0.01),VIP分布明显增加(P<0.05或P<0.01)。结论:大黄对大鼠胃运动有明显的抑制作用,其机制可能与胃窦肌间神经丛SP的分布减少及VIP的分布增加有关。
Objective: To study the mechanisms of rheum officinale for gastric motility promotion. Methods: All 32 Wistar rats were randomly divided into rheumoffcinale group and control group and then were given rheum offcinale decoction or distilled water. After 1 and 6hours respectively, the gastric emptying changes of rats were assayed by Dextran blue-2000 being as a indicator; distributions of substance P (SP) and vasoactive intestinal peptide (VIP) in antral myoplexus were observed by immunohistoehemistry.Results: The gastric emptying of rats was markedly decreased (P<0. 01 or P<0.05), the distributions of SP positive nerves decreased significantly and the distributions of VIP positive nerves increased (P<0. 01 or P<0.05) in antral myoplexus at 1, 6hours after rheum officinale decoction was given. Conclusion: The restraining effects of rheum officinale on gastric motility in rats may be corelated to the decreased distrbutions of SP and increased distributions of VIP in gastric myoplexus.
出处
《福州总医院学报》
2001年第3期156-157,共2页
Journal of Fuzhou General Hospital
