摘要
为证实褪黑素受体 (melatoninreceptor ,MR)存在于人肌肉组织内 ,取人脊柱两侧肌肉组织抽提总RNA ,应用RT -PCR检测MRmRNA并测序。结果表明人脊柱两侧肌肉组织内有褪黑素受体mt1及MT2 表达 ,测序结果与人类褪黑素受体cDNA相吻合。证明肌肉组织是褪黑素作用的靶器官 ,褪黑素可能通过存在于肌肉组织中的mt1及MT2 受体 ,来调节脊柱两侧肌肉组织的生长发育。
To verify the expression of melatonin receptor mRNA in human, muscle, muscle beside vertebrae was collected to obtain total RNA and the mRNA of melatonin receptor was detected by RT-PCR method.The electrophoretic results of RT-PCR products by mt 1 and MT 2 primer were all positive and the sequence is corresponding with human melatonin receptor cDNA. It suggests that melatonin may act on the muscle beside vertebrae directly and regulate its growth and development.
出处
《标记免疫分析与临床》
CAS
2004年第4期212-214,共3页
Labeled Immunoassays and Clinical Medicine
关键词
褪黑素
受体
逆转录-聚合酶链反应
肌肉组织
Melatonin
Receptor
Reverse transcript-polymerase chain reaction
Muscle
