摘要
以玉米 (Zeamays)鲁单 5 0幼苗为实验材料 ,采用等渗的离子胁迫 (0 8%NaCl - 0 6MPa)和非离子胁迫 (2 0 %PEG)模拟渗透胁迫处理 ,从受胁迫的玉米幼苗根系中分离出 6 3 5kD热稳定蛋白。用ABA、Ca2 + 、Ca2 + 螯合剂EGTA、CaM抑制剂TFP处理幼苗 ,96h后取材进行SDS PAGE电泳。结果表明 ,该蛋白既可被渗透胁迫 ,也可被ABA诱导产生 ,且两者同时处理时 ,该蛋白的表达量更大 ;Ca2 + 促进该蛋白的表达 ;Ca2 + 螯合剂EGTA对该蛋白的表达有抑制作用 ;CaM抑制剂TFP对该蛋白的表达也有抑制作用 ,且高浓度TFP对非离子胁迫下的作用比离子胁迫时明显。说明 6 3 5kD热稳定蛋白受Ca2 + CaM信号传导途径的调控。
The maize(Zea mays) seedlings were cultivated by Hoagland solution with simulated isotonic ionic and nonionic osmotic stress by NaCl(0.8%) and PEG(20%), respectively.A 63.5 kD heat-stable protein was separated from the roots. Under different treatments, the analysis of heat-stable proteins by SDS-PAGE showed that the 63.5 kD protein was induced by osmotic stress or ABA.Moreover, the protein subunit content induced by combination of osmotic and ABA was more than that by individual of them.The expression of the induced protein was enhanced by Ca 2+ and inhibited by EGTA and TFP.The regulation by high-concentration TFP was more obvious under nonionic stress than under ionic stress.The results suggest that the expression of the 63.5 kD heat-stable protein is regulated by Ca 2+ / CaM messenger system.
出处
《作物学报》
CAS
CSCD
北大核心
2005年第1期83-87,共5页
Acta Agronomica Sinica
基金
国家重点基础研究发展规划项目 (G19990 1170 0 )
国家自然科学基金 (3 9870 5 2 6
3 0 4710 5 2 )资助
关键词
玉米
渗透胁迫
热稳定蛋白
信号物质
Maize
Osmotic stress
Heat-stable protein
Signal substances
