摘要
目的:探讨海带多糖(LJP)抗辐射作用与淋巴细胞凋亡的关系。方法:将Wistar雄性大鼠随机分为对照组,模型组和4个不同剂量LJP实验组(100、200、300、400mg/kg),每组6只。对照组,模型组每只给予2ml生理盐水,实验组给予不同浓度等量LJP溶液。灌胃10d后模型组和实验组行一次性全身Coγ射线照射,每只剂量9.0Gy,18h后测定各组大鼠脾脏淋巴细胞增殖反应;60腹腔巨噬细胞数目及吞噬功能;血清溶血素;迟发性变态反应及脾淋巴细胞凋亡率和凋亡相关基因Bcl-2和Bax蛋白的表达。结果:LJP各实验组与模型组比较,能非常显著地对抗辐射对相关免疫指标的抑制作用(P<0.01)。TUNEL实验与流式细胞术均说明LJP对照射诱导的大鼠脾淋巴细胞凋亡具有一定的拮抗作用,并呈现浓度的依赖效应。免疫组化结果显示:随着LJP含量的增加,照射后脾淋巴细胞的Bcl-2蛋白表达增加而Bax蛋白表达下降,Bcl-2/Bax上升。结论:LJP抗辐射作用的可能机制之一是使凋亡抑制基因Bcl-2表达上调、促凋亡基因Bax蛋白表达下调,Bcl-2/Bax比值明显增大,防止脾淋巴细胞凋亡,促进免疫功能恢复。
Objective:To investigate the relation of antiradiation effect of LJP and lymphocyte apoptosis. Methods:36 Wistar rats were randomly divided into 6 groups (n=6): control, model,and LJP given i.g.at 4 doses(100,200,300,400 mg/ kg bw) for 10d before whole-body irradiation with 9.0 Gy Coγ-ray. 18h later,the effects of LJP on the indices of immune function of the irradiated rats 60 were measured. TUNEL and flow cytometry were used to study the effects of LJP on splenic lymphocyte apoptosis and immunohistochemical technique was used to detect the expression of Bcl-2 and the Bax protein. Results:LJP significantly modulated immune function in irradiated rats. The apoptosis ratio of splenic lymphocyte of the model group was higher than those of other groups. LJP could markedly inhibit the effects of irradiation on apoptosis and increase the ratio of bcl-2/bax protein in dose-effect manner. Conclusion:LJP could inhibit splenic lymphocyte apoptosis induced by irradiation, and its mechanism is associated with regulating the expression of bcl-2 and bax protein of splenic lymphocyte. Key word:laminaria japonica polysaccharides; irradiation; lymphocyte; apoptosis apoptosis-related genes
出处
《营养学报》
CAS
CSCD
北大核心
2004年第6期471-473,共3页
Acta Nutrimenta Sinica
基金
湖北省科技厅重点攻关项目资助(2001AA307B)
关键词
海带多糖
辐射
淋巴细胞
凋亡
凋亡相关基因
laminaria japonica polysaccharides
irradiation
lymphocyte
apoptosis apoptosis-related genes
