摘要
本文采用615近交系小鼠肝癌腹水瘤H_(ca)-F_(25)/CL-A_2细胞,测定钙拮抗剂异搏定作用前后磷脂酰肌醇特异的磷脂酶C(PI-PLC)活性的变化,并与同样处理的瘤株内钙恒稳的有关指标相比较。结果表明经异搏定处理后的A_2细胞PI-PLC活性显著降低,并与钙恒稳的指标基本呈现相应的平行变化趋势。提示PI-PLC可能参与肿瘤细胞内钙恒稳的变化过程,异搏定的作用可能与磷脂酰肌醇信号传导系统相关。
H_(ca)-F_(25)/CL-A_2, a type of ascites hepatoma cell clones of 615 inbreeding strain mice was employed and the activities of phosphatidylinositol-specific phospholipase C (PI-PLC) were measured before and after treated with verapamil, and compared with some indexes of intracellular calcium homeostasis. The results showed that PI-PLC activities were decreased in the tumor cells after treated with verapamil. These changes occurred simultaneously with those changes indexes of calcium homeostasis. The results suggested that PI-PLC involved in changing the process of intracellular calcium homeostasis, and the effect of verapamil was closely associated with the phosphoinositide signaling pathway in the tumor cells.
关键词
磷脂酰肌醇
磷脂酶
异搏定
肝肿瘤
Phosphatidylinositol-specific Phospholipase C (PI-PLC)
Verapamil
Calcium homeostasis
Tumor differentiation
Ascites hepatoma
