摘要
鹅副粘病毒分离株NA_1经 11日龄鸡胚增殖后纯化。提取病毒的基因组RNA ,采用RT_PCR扩增出与预期设计的 1 7kb大小相符合的特异条带。将扩增产物提纯后克隆入pMD 18_T载体 ,经纯化、筛选及酶切鉴定后 ,初步获得了含鹅副粘病毒HN基因的阳性克隆 ,并对阳性克隆进行测序。测序后拼接得出HN基因的全序列长度为 174 0bp ,该基因的ORF总长为 1716bp ,编码 5 71个氨基酸。与GenBank下载的 15株参考毒株比较HN基因编码区全核苷酸序列 ,发现所测NA_1毒株与参考毒株YG97核苷酸序列同源性为 97 3% ,与F48E9同源性为 84 5 % ,与LaSota为 82 2 %。
Avian paramyxovirus strain NA-1 isolated from goose was propagated in 11-day-old chicken embryos.The genomic RNA of NA-1 were extracted.The HN gene of the virus has been successfully amplified by RT-PCR,and further cloned into pMD 18-T vector,and a positive recombinant plasmid was obtained by restriction enzyme analysis. The complete sequence of HN gene was obtained finally and the nucleotide sequence of the HN gene was 1 740 bp.The sequence analysis demonstrated that 15 reference strains loaded down from GenBank,the result of sequence analysis indicated that NA-1 strain exhibited 97.3?% homology with reference strain YG 97,84.5?% homology with strain F-(48)E-9 and 82.2?% homology with strain La Sota. The results indicated the mutation of the HN gene between the goose's paramyxovirus and NDV varied to a great extent.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2005年第1期18-21,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
吉林省科委发展计划项目 (2 0 0 0 0 0 2 0 0 )
