摘要
目的 建立三代繁殖低硒大鼠动物模型 ,为了进一步系统研究低硒对大鼠脑神经智能、心血管、骨软骨、免疫器官 ,生长发育以及生育、衰老的影响提供研究用动物模型。方法 利用低硒酵母为主要原料人工配制半合成饲料喂养大鼠并连续繁殖到第三代 ,历时一年半。原代动物选用健康断乳后封闭群SD大鼠6 0只 ,体重平均值为 (5 0± 5 )g。作为F0代 ,五组大鼠分别为 1 合成饲料对照组 (硒水平 0 1～ 0 3μg g,碘水平≥ 0 2 μg g) ,2低硒一组 (硒水平 0 0 2 μg g,碘水平≥ 0 2 μg g) ,3 低硒二组 (硒水平 0 0 0 5 μg g ,碘水平≥0 2 μg g) ,4 低碘组 (硒水平 0 1～ 0 3μg g ,碘水平 0 0 4 μg g) ,5 低硒低碘组 (硒水平 0 0 1μg g ,碘水平0 0 4 μg g) ;雌雄分笼饲养。F1、F2、F3的分组为 :取消低硒二组 ,低硒低碘组饲料中硒水平同单纯低硒组、碘水平同单纯低碘组 ,余同原代。雌雄分笼饲养。每代大鼠检测血清硒水平、全血GPX活性 ,血清总T3、T4、TSH水平 ,同时对各代仔鼠生长发育期体重、分娩数、新生鼠存活数等进行了观察。结果 发现人工半合成低硒饲料可持续保持大鼠低硒状态 ,使血清硒值在三代繁殖大鼠中维持低水平 ;全血GPX活性保持低水平 ;低硒大鼠分娩数、新生仔鼠存活率下降 ,体重减?
Objective To established selenium deficiency of the breed third generation SD rats animal model in series. Methods A Se-deficient diet mainly based on Se-deficient yeast. Groups of six female and six male SD rats original generation (F0) were fed on one of the five diets: (1) selenium- and iodine-adequate control group (Se+I+ group. Selenium:0.1-0.3μg/g,iodine ≥0.2μg/g), (2) selenium-deficient iodine-adequate group 1(Se-I+ group. Selenium: 0.02μg/g ,iodine≥0.2μg/g), (3)selenium-deficient iodine-adequate group 2(Se-I+ g roup2 Selenium:0.005μg/g,iodine ≥0.2μg/g), (4) selenium- adequate iodine- deficient group(Se+I- group. Selenium:0.1-0.3μg/g,iodine:0.04μg/g), (5) selenium- and iodine- deficient group(Se-I- group. Selenium:0.01μg/g,iodine :0.04μg/g). Selenium-deficient iodine-adequate group 2 had been deled since generation 1 (F1). Serum Se and thyroid hormone concentrations, whole blood glutathione peroxidase (GSH-Px) activities, the weight, childbearing and livability were examined. Results There were durative lower Se concentrations in serum and lower GSH-Px activities in blood, and the weight, childbearing and livability reduced in Se- group and Se-I- group From F0 to F3. Conclusion We have established prolonged selenium deficiency of the SD rat animal model breed third generation in series successfully.
出处
《卫生研究》
CAS
CSCD
北大核心
2004年第6期705-710,共6页
Journal of Hygiene Research
基金
国家自然科学基金资助项目 (No .30 1 70 832 )
广东省十五攻关项目 (No.A1 0 80 2 0 3)
省教育厅"千百十工程"优秀人才培养基金
关键词
低硒
大鼠
动物模型
selenium deficiency, rat,animal model
