摘要
目的 研究p ,p′ DDE对离体培养大鼠睾丸支持细胞内信号转导通路的影响。方法 从大鼠睾丸组织中分离支持细胞进行离体原代培养 ,以SABC方法来检测细胞内p ERK的表达。结果 发现支持细胞细胞内p ERK随p,p′ DDE剂量的增高而染色加深 ,而且胞浆胞核都表达。结论 p ,p′ DDE可导致支持细胞内ERK磷酸化 ,诱导ERK下游通路的激活 ,从而影响到基因的转录和表达。
Objective To study effects of p,p′-DDE on intracellular p-ERK of rat sertoli cells in vitro. Methods We cultulred sertoli cells separated from testicular tissue of rats and examined the changes of intracellular p-ERK, using immunohistochemical SABC method. Results It was showed that intracellular p-ERK increased with the dose of p,p′-DDE and had a dose-dependent trend. Conclusion The results suggested that p,p′-DDE could result in the phosphorylation of ERK and induce the activation of the downstream road and the transcription and expression of genes.
出处
《卫生研究》
CAS
CSCD
北大核心
2004年第6期668-670,共3页
Journal of Hygiene Research
基金
国家 973项目资助 (No .2 0 0 2CB51 2 90 8)
