摘要
目的:研究编码嵌合体SBR-CTDA1基因片段转化盐藻受体的方法及影响条件。方法:①观察不同时间及功率超声波对盐藻生长的影响;②用含有目的基因(编码嵌合体SBR-CTDA1基因片段)和标记基因(bar基因)的质粒pROSB进行盐藻的超声波法转化和农杆菌共培养法转化。结果:①55w5min和88w3min盐藻生长较好;②经超生波法转化获得藻落中随机提取三个盐藻的总DNA,通过PCR分析,证明目的基因已整合进一个盐藻的基因组;农杆菌共培养法转化结果不理想。结论:初步建立了可食用盐藻防龋疫苗的转化体系,获得转基因阳性藻落,为制备口服疫苗奠定良好基础。
Objective: The propose is to study the transformation methods and influential factors of transformingDunaliella salina. with the gene encoding chimera SBR-CTDA1 Methods: 1.Observed the effects of different time spansand power of ultrasonic wave on the growth of Dunaliella salina. 2.Plasmid pROSB containing target gene(SBR-CT) andreport gene(bar) was transfected into D.salina with ultrasonic wave method and co-culturing with Agrobacterium. Results:① Dunaliella salina growth well in 55w 5min and 88w 3min. ② Total DNAs were extracted randomly from 3 vigorousalgal clones transformed with in ultrasonic wave method . PCR results showed that target gene has been integrated intoore genome of the three D.salina cones; results of Agrobacterium tumefaciens co-cultivationwere not well. Conclusion:The transformation system was preliminarily built up and transgenic D.salina containing target gene was obtained, whichmay lay a foundation for edible vaccines against caries.
出处
《中华老年口腔医学杂志》
2003年第3期140-143,共4页
Chinese Journal of Geriatric Dentistry
