摘要
应用高效液相色谱法 ,测定了不同产地补骨脂中补骨脂素和异补骨脂素的含量。色谱柱为Kromasil C18( 4 6mm× 2 5 0mm ,5 μm) ,流动相 :甲醇 水 ( 4 5∶5 5 ) ,检测波长 :2 4 5nm ,流速 :1 0ml/min ,柱温 :35℃。结果 :补骨脂素在 0 0 4 4 8～ 0 4 4 8μg(r =0 9999)范围内、异补骨脂素在 0 0 35 6～ 0 35 6 μg(r =0 9997)范围内线性关系良好 ,补骨脂素和异补骨脂素平均回收率分别为 98 6 9% (RSD =1 97% )和 10 1 0 9% (RSD =3 0 6 % )。
Objective: To study the psoralen and isopsoralen content in Psoralea corylifolia from different habitats. Method: C 18 column was used. Mobile phase was methanol-water (45∶55).The detection wavelength was at 245nm and the flow rate was 1.0ml/min. Result: The linearity was obtained over the range of 0.0448～0.448μg(r=0.9999) for psoralen,0.0356～0.356μg(r=0.9997) for isopsoralen.Conclusion: The contents of psoralen and isopsoralen in Psoralea corylifolia from different habitats have no obvious difference.
出处
《中药材》
CAS
CSCD
北大核心
2004年第11期817-818,共2页
Journal of Chinese Medicinal Materials
