摘要
胃动素是一种重要的调节胃肠运动功能的脑肠肽,又称运动激素,体内分布广泛,除胃肠粘膜外,还存在于中枢和外周的神经组织中。脾虚证本质研究发现脾虚失健运的诸多病理生理表现均与胃肠功能紊乱有关。因此本文同步地从MOT含量和MOT内分泌细胞两方面观察了脾虚模型大鼠与MOT的关系。目的:探讨“脾虚失健运”与MOT的关系。方法:用RIA法对脾虚造模7天和14天大鼠血浆、下丘脑、十二指肠、空肠中MOT含量进行测定,同时用SABC法对十二指肠粘膜和下丘脑组织中MOT内分泌细胞进行染色。结果:造模7天大鼠模型组织、血浆中MOT含量升高,Mo细胞数下降,强阳性细胞率升高,下丘脑MOT视经元数升高(P<0.01,P<0.05);造模14天组织、血浆中MOT含量下降,Mo强阳性细胞率升高,下丘脑MOT神经元数下降(P<0.01,P<0.05)。结论:MOT含量及其内分泌细胞的形态和数量在脾虚证病程中呈动态改变。
Objective: To investigate the relationship of 'dysfunction of the spleen in transport' with motilin. Methods: The short - term and long - term rat models of spleen - asthenia syndrome (RMS A Ⅰ and RMSA n group) were treated by re-serpine for seven days and fourteen days respectively. Sijunzi decoction was used as a remedy to verify the availability of the model. Immunohistochemical SABC method was employed to stain Mo cell of duodenum and MOT neuron in hypothalamus. The contents of motilin in tissues and plasma of the rat models and normber rats were measured by RIA. Results : In the rat models of seven days, as compared with controls, the number of Mo cell reduced ( P <0.01) ,the percentage of strong positive Mo cell increased ( P <0.05) ; the number of MOT neuron increased ( P <0. 01) ; both tissues and plasma had higher levels of MOT than those of controls ( P <0.05, P <0.01). In the rat models of fourteen days, as compared with controls, the number of MOT neuron reduced ( P < 0.01) , the percentage of strong positive Mo cell reduced ( P < 0. 01) ; both tissues and plasma had lower levels of MOT than those of controls ( P <0.05, P <0.01). Conclusions : The contents of motilin and the appearance and quantity of the Mo cells, MOT neuron in hypothalamus varyed with the development of spleen - Asthenia syndrome.
出处
《中医药学刊》
2004年第11期2028-2030,共3页
Study Journal of Traditional Chinese Medicine
基金
国家自然科学基金资助课题(39770886.39670865)
关键词
脾虚证
胃动素
内分泌细胞
神经元
Spleen - asthenia syndrome
Motilin
Neuron
enteroendocrine cell
