摘要
在构建内蒙古阿尔巴斯白绒山羊次级毛囊兴盛期皮肤组织cDNA文库的基础上 ,随机挑取 6 36个克隆从 5’端开始测序 ,对序列进行特征分析。分析结果表明有 4 1个ESTs与绵羊KAP6 - 1基因同源性大于 95 % ,且期望值小于le - 10 ,进一步分析可被分为 6个Clusters。从每个Cluster中挑取一个全长cDNA序列 ,其核苷酸序列和预测编码的蛋白质序列表明AY310 75 3与绵羊KAP6 - 1最为相似、AY310 75 0差别最大 ,AY310 75 1和AY310 75 2在编码区分别缺少 36个的核苷酸或 12个的氨基酸。
Under the basic of constructing skin cDNA library during secondary follicle anagen,636 clones were measured sequences from 5' end.Aligning with Blastn database of Genebank,the similarity of 41 ESTs to sheep KAP6-1 was more than 95% and the expectation was less than 1e-10.Further analysis 41 full length cDNAs of KAP6 could be classified 6 different clusters.Choosing a full cDNA from every cluster and comparing their nucleotide acid and forecasted protein sequence,AY310753 was same to sheep KAP6-1,but AY310750 has the most difference.AY310751 and AY310752 are lack of 36 nucleotide acids or 12 amino acids in the coding division.
出处
《生物技术》
CAS
CSCD
2004年第5期8-10,共3页
Biotechnology
基金
国家自然科学基金地区重点项目 (399690 0 2 )资助
关键词
绒山羊
CDNA文库
遗传
基因
内蒙古
Cashmere goat
cDNA library
characteristic analysis
ESTs
KAP6-1
