摘要
AIM: To investigate the effect of histone acetylation on regulation of p21^WAF1 gene expression in human colon cancer cell lines.METHODS: Two cell lines, Colo-320 and SW1116 were treated with either trichostatin or sodium butyrate.Expressions of p21^WAF1 mRNA and protein were detected by real-time RT-PCR and Western blotting, respectively.Acetylation of two regions of p21^WAF1 gene-associated histones and total cellular histones were examined by chromatin immunoprecipitation assay and Western blotting.RESULTS: Trichostatin or sodium butyrate re-activated p21^WAF1 transcription resulted in up-regulated p21^WAF1 protein level in colon cancer cell lines. Those effects were accompanied by an accumulation of acetylated histones in total cellular chromatin and p21^WAF1 gene-associated region of chromatin.CONCLUSION: Histone acetylation regulates p21^WAF1 expression in human colon cancer cell lines, Colo-320 and SW1116.
AIM:To investigate the effect of histone acetylation on regulation of p21^(WAF1) gene expression in human colon cancer cell lines. METHODS:Two cell lines,Colo-320 and SW1116 were treated with either trichostatin or sodium butyrate. Expressions of p21^(WAF1) mRNA and protein were detected by real-time RT-PCR and Western blotting,respectively. Acetylation of two regions of p21^(WAF1) gene-associated histones and total cellular histones were examined by chromatin immunoprecipitation assay and Western blotting. RESULTS:Trichostatin or sodium butyrate re-activated p21^(WAF1) transcription resulted in up-regulated p21^(WAF1) protein level in colon cancer cell lines.Those effects were accompanied by an accumulation of acetylated histones in total cellular chromatin and p21^(WAF1) gene-associated region of chromatin. CONCLUSION:Histone acetylation regulates p21^(WAF1) expression in human colon cancer cell lines,Colo-320 and SW1116.
基金
Supported by the National Natural Science Foundation of China,No.30170413 and Foundation for the Authors of National Excellent Doctoral Dissertation of China,No.199946 and Foundation of Shanghai Education Committee,No.02SG45
