摘要
目的 通过检测急性心肌梗死 (acutemyocardialinfarceion ,AMI)患者血清环氧化酶 - 2 (COX- 2 )活性 ,探讨COX - 2与AMI病情的关系。方法 将样本分为AMI组和正常对照组 ,AMI组均于急性发病后 1 (治疗前 )、 2、 3、 7、 1 4d各抽一次空腹肘静脉血 2 0ml:正常对照组从体检健康人群中抽空腹肘静脉血 2 0ml,酶联免疫吸附双抗体夹心法检测血清COX - 2的活性。结果 COX - 2活性在AMI死亡组明显高于存活组 (P <0 0 5 ) ;AMI组COX - 2活性在 1、2、 3d明显高于正常对照组 (P均小于 0 0 1 ) ,于 7、 1 4d它们与对照组比较则均差异显著性 (P >0 0 5 ) ;且COX - 2的活性变化与AMI患者的血清CK -MB含量的变化、临床积分、梗死面积均呈明显的正相关性。结论 COX - 2活性与AMI患者的病情变化相一致 ,随病情的严重程度增加而升高 ,病情减轻而下降 ,提示COX - 2可能参与了AMI的病理生理过程 ,COX -
Objective To detect the changes of serum COX-2 activity in acute myocardial infarction(AMI)and try to find out the relationship between COX-2 activity and condition of patients with AMI. Methods All samples were divided into two groups: AMI group and normal control group.2 ml of venous blood was drawn on the first day, 2nd day, 3rd day, 7th day and 14th day in AMI group. 2 ml of fasting venous blood was collected from healthy check-up persons forming the control group. ELISA KIT measured the serum concentration of COX-2 activity. Results The serum level of COX-2 was significantly different between those who survived and those who died from AMI( P <0 05).The levels of COX-2 activity in the contvol group was significantly lower than those in AMI group on days 1,2 and 3 post attack(All P <0 01). However, on both the 7th and 14th day after AMI,the level of COX-2 declined markedly in the patientsso that there was no more statistical significant difference compared to the control group (both P >0.05).There were significant positive correlations between serum COX-2 activity and the CK-MB level, the clinical scores and the infarction size of AMI patients. Conclusion The changes of the COX-2 level were consistent with the clinical development of AMI, which increase when patientscondition deteriorated. These suggested that COX-2 activity would probably become one of the monitoring and prognostic factors for AMI patients.
出处
《中华急诊医学杂志》
CAS
CSCD
2004年第8期539-541,共3页
Chinese Journal of Emergency Medicine
