摘要
目的:探讨成骨蛋白-1(OP-1)在脊柱融合术中的作用。方法:选用12只成年猕猴接受腰椎融合术,实验组、对照组分别采用OP-1加自体骨、单纯自体骨植骨。术后当日和术后4、8、12、16、20周分别拍腰椎正位片,观察植骨生长的情况。术后12周和20周分批处死动物,取脊柱标本进行生物力学和组织学研究。结果:术后第12周X线检查发现,OP-1组中,3只猕猴获双侧牢固融合,2只单侧牢固融合,1只部分融合;对照组只有1只获单侧牢固融合。实验组的植骨融合效果明显优于对照组,而且融合时间也明显提前,但两组的扭转刚度无显著性差异(P>0.05)。组织形态学评估发现,实验组的总平均成骨值为0.87±0.10,对照组为0.45±0.20,两组的成骨值有显著性差异(P<0.01),实验组的成骨活性明显高于对照组。结论:在无脊柱内固定的条件下,OP-1对脊柱后外侧融合具有显著的促进作用,可提高融合效率,缩短融合时间。
Objective: To determine the efficacy of osteogenic protein-1(OP-1) used in posterior lumbar spinal fusion.Method:12 Rhesus monkeys were randomly divided into two groups:experimental group and control group.Every animal was undergone a surgical procedure intended to produce bilateral posterolateral fusion of the lumbar spine.Experimental group used OP-1/CMC(carboxymethylic cellulose) with autologous bone graft for fusion and control group used CMC with autologous bone graft.Clinical observations,radiological analysis,biomechanical testing and histological evaluation were performed postoperatively.Result:The radiographic data at 12 weeks post-operation demonstrated that six animals in the experiment group had evidence of bone growth,three of them got solid bilateral fusion,two got solid unilateral fusion.But in the control group,only one animal got solid unilateral fusion,none got solid bilateral fusion.The statistic analysis showed that the two groups had significant difference (P<0.01).Bone formation activity was quantified in the way of osteo-formation value(OFV).Average OFV of experimental group was 0.87±0.10 and that of control group was 0.45±0.20.There was significant difference between the two groups(P<0.01).Conclusion:The results indicate that rhOP-1 used without internal fixation can significantly promote the efficacy of bilateral lumbar spine fusion,shorten the fusion time,and cause no side-effect.
出处
《中国脊柱脊髓杂志》
CAS
CSCD
2004年第11期673-675,i001,共4页
Chinese Journal of Spine and Spinal Cord
关键词
成骨蛋白—1
腰椎融合术
Osteogenic protein-1
Lumbar spine fusion
