摘要
目的 观察米非司酮对子宫腺肌病灶组织细胞的作用。 方法 采用噻唑兰法观察米非司酮对 11例子宫腺肌病患者腺肌病灶组织原代培养细胞体外生长状态的影响 ,实验分为 1.2 5、2 .5、5 0mg/L 3个药物浓度组 ,培养 5 2h ,测定OD值 ,并与空白对照组对比。 结果 1.2 5、2 .5、5 0mg/L组及对照组的OD值分别为 (0 .2 19± 0 .0 4 4 )、(0 .185± 0 .0 38)、(0 .15 2± 0 .0 33)、(0 .2 6 2± 0 .0 6 8) ,5 0与 1.2 5mg/L组有显著差异 (P <0 .0 1) ,5 0、2 .5mg/L组与对照组有显著差异 (P <0 .0 1)。细胞生长抑制率 2 .5mg/L组 (IR =2 9.4 %)是 1.2 5mg/L组 (IR =16 .4 %)的 1.79倍 ,5 0mg/L组 (IR =4 2 .0 %)是 2 .5mg/L组的 1.4 3倍。 结论 米非司酮能够抑制子宫腺肌病腺肌组织细胞的生长 ,其抑制能力与药物浓度呈正相关。
Objective: To observe the effects of mifepristone on the cultured adenomyotic cells. Method:With MTT assay, the growth state of primary cell culture from adenomyotic implants of 11 women with adenomyosis was studied. The cells of each case were divided into four groups, three treatment groups (1.25 mg/L, 2.5 mg/L, 5.0 mg/L mifepristone, respectively) and one control group, cultured for 52 hours before the OD value was determined. Results: The OD values of 1.25 mg/L, 2.5 mg/L, 5.0 mg/L mifepristone and control group were (0.219±0.044),(0.185±0.038), (0.152±0.033) and (0.262±0.068) respectively. The OD value of 5.0 mg/L group was significantly lower than that of 1.25 mg/L group (P<0.01), and both 5.0 mg/L and 2.5 mg/L groups had significantly lower OD values than the control group (P<0.01). The inhibition rate of cell growth in 2.5 mg/L group (IR=29.4%) was 1.79 times as much as that in 1.25 mg/L group (IR= 16.4%), while in 5.0 mg/L group (IR=42.0%)it was 1.43 times as much as that in 2.5 mg/L group. Conclusion: Mifepristone could suppress the growth of adenomyotic cells in vitro in a dose-dependent manner.
出处
《生殖医学杂志》
CAS
2004年第5期270-272,共3页
Journal of Reproductive Medicine
基金
甘肃省科学事业费资助 (QS0 0 2 C3 3 0 11)
