摘要
目的探讨地塞米松对哮喘患者单核细胞凋亡状态及其分泌细胞因子的影响作用。方法采用Ficoll-Hypaque密度梯度离心及贴壁培养法对30例支气管哮喘患者和15名健康对照者单核细胞进行分离、纯化后,以空白组、地塞米松组培养24h,经琼脂糖凝胶电泳、流式细胞仪检测细胞凋亡的发生率,同时以酶联免疫吸附试验(ELISA)检测细胞培养上清液中粒细胞—巨噬单核细胞集落刺激因子(GM-CSF)、肿瘤坏死因子(TNF)-α水平。结果哮喘组单核细胞自然凋亡率明显低于对照组(P<0.01),分泌TNF-α、GM-CSF较对照组明显增高(P<0.01)。地塞米松作用后,哮喘组单核细胞凋亡率明显增加(P<0.05);细胞因子分泌水平下降,两组间比较差异无显著性(P>0.05)。结论地塞米松促进哮喘患者单核细胞凋亡并抑制其分泌前炎性细胞因子TNF-α、GM-CSF可能是其治疗支气管哮喘的机制之一。
Objective To investigate the effect of dexamethasone on cytokines secreted by monocyte and monocyte apoptosis in bronchial asthma (BA). Methods Monocytes from 30 asthma patients and 15 normal (NL) donors were isolated by density centrifugation with Ficoll-Hypaque and adherent incubation, then were cultured with or without dexamethasone. After a 24-hour incubation, granulocyte macrophage colony-stimulating factor (GM-CSF),tumor necrosis factor-α (TNF-α) were measured in the culture supernatants by an enzyme-linked immunosorbent assay (ELISA) . Apoptotic cells were analysed by DNA electrophoresis and flow cytometry. Results The basal production of GM-CSF and TNF-α was higher in the monocyte of BA individuals (P<0.01), cultures of BA monocytes exhibited a significantly lower apoptosis rate than the NL monocytes (P<0.01). After stimulated by dexamethasone,the production of cytokines decreased; there was no significant difference between the two groups, apoptosis of BA monocytes enhanced (P<0.05). Conclusion Dexamethasone may have an effect for inhibiting the production of pro-inflammatory cytokines GM-CSF and TNF-α of monocytes, and enhancing the monocyte apoptosis in BA.This may become one of its antiasthmatic mechanisms.
出处
《中国药物与临床》
CAS
2004年第10期760-762,共3页
Chinese Remedies & Clinics
