摘要
研制以纳米金颗粒与聚乙烯缩丁醛(PVB)及葡萄糖氧化酶(GOD)混合制成复合固酶膜基质,制成葡萄糖氧化酶生物传感器。研究了使用硫堇(Thi.)作为电子媒介体对葡萄糖氧化酶电极响应的影响。实验证明,电子媒介体的引入可以大大提高葡萄糖氧化酶电极响应的灵敏度,为制备有实用价值的葡萄糖生物传感器提供了重要的实验依据。
A novel glucose sersor has been contrived by using Au colloid modified electrode with thionine as a mediator. Glucose oxidase (GOD) had been adsorbed to colloidal gold sols combined with polyvinyl butyral(PVB), which were immobilized onto the surface of a platinum electrode. The GOD immobilized on colloidal gold was stable and retained its functional activity. Thionine is utilized as a mediator to facilitate efficient electron transfers between the electrode surface and glucose oxidase. The response characteristics and optimization of the bioeletrode design are evaluated. The hiosensor substantially improved sensitivity to the glucose. The oxidation current investigated with cyclic vohammetric peak current was linear with glucose concentration ranging from 2.5×10^(-5) mol/L to 5.0×10^(-3) mol/L with a limit of 1.3×10^(-5) mol/L. The anti-interference and application of the biosensor in human serum were excellent.
出处
《化学传感器》
CAS
2004年第3期52-55,共4页
Chemical Sensors
基金
教育部优秀青年教师资助项目(教人司(2002)40)
重庆市自然科学基金资助课题(2002-7477)
