摘要
本研究参考EPA1623的三个关键步骤:原虫过滤浓缩→分离→染色鉴定,对原虫的检测方法在过滤、浓缩和染色等过程中进行了改进,并用膜过滤-梯度分离-Giem Sa染色、膜过滤-IMS-GiemSa染色及囊过滤-IMS-IFA(EPA1623)三种检测方法对原虫的染色特征,形态结构和回收率的检测进行了方法学的探讨和比较分析。结果显示:三种检测方法G的回收率均在EPA规定的质控范围内,而膜-梯度分离-Giemsa和膜-IMS-Giemsa的C的回收率略低于EPA质控范围。但将EPA方法中的滤囊过滤改成滤膜过滤,免疫荧光染色改成GiemSa染色,能减少成本费用约60%-90%,而且实验条件要求较低,可以用于自来水出厂水和污水(不经膜过滤)中原虫的筛选。
In this study,three methods according to EPA1623 were used for detection of Giardia and cryptosporidium in water.The results showed that the r ecovery obtained by the 3 methods was within the quality - control range. The chang e of filtering cyst into filtering membrane and immunofluorescent staining into GiemSa staining can reduce cost for 60 - 90% . The method can be widely used for det ection of Giardia and cryptosporidium in water.
出处
《中国卫生检验杂志》
CAS
2004年第5期549-550,共2页
Chinese Journal of Health Laboratory Technology
