摘要
目的 :制备并纯化抗核糖核酸酶抑制因子 (RI)的抗体。方法 :从人胎盘中提取RI,经亲和层析纯化后免疫家兔制备RI抗体。用rProteinASepharoseFastFlow层析对抗RI抗体进行纯化 ,并用SDS PAGE、ELISA及Westernblot等进行鉴定。结果 :制备并纯化了抗RI抗体 ,经SDS PAGE分析达到了电泳纯。结论 :获得了效价高、特异性强、稳定性好的抗RI抗体 。
AIM: To prepare and purify the antibody against ribonuclease inhibitor(RI). METHODS: RI was extracted from human placenta and purified by affinity chromatography. Rabbits were immunized with RI, anti-RI antibody was got and then purified through rProtein A Sepharose Fast Flow chromatography column. The characters of the antibody was identified by SDS-PAGE, ELISA and Western blot. RESULTS: An anti-RI antibody was obtained and purified. SDS-PAGE analysis showed that the purified anti-RI antibody has high purity. The results of Western blot and ELISA indicated that anti-RI antibody had high specificity and good stability. CONCLUSION: The anti-RI antibody with high titer, high specificity and good stability has been acquired, which lays the foundation for further research on RI.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2004年第5期572-574,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助项目 (No .39870 2 4 2 )
关键词
核糖核酸酶抑制因子
抗体
亲和层析
纯化
ribonuclease inhibitor
antibody
affinity chromatography
purification
