摘要
利用农杆菌介导法,研究了辐照、愈伤组织状态、共培养条件及负压等辅助处理对结缕草愈伤组织转化频率和存活率的影响。结果表明:2J/kg的辐照剂量有利于提高转化频率;1～2个月的愈伤组织适于转化;全光照的共培养条件下转化频率高于16h光照培养和8h暗培养条件下的转化频率;农杆菌与愈伤组织混合培养时给予适当的负压条件可提高愈伤组织转化频率和存活率。
The assistant method of agrobacterium-mediated transformable efficiency which includes irradiation,callus,co-cultivation and negative pressure,is explored with the callus of Japanese lawngrass(Zoysia japonica Steud.).The results are as follow:2?J/kg of irradiation-dosage benefits the exaltation of transformable efficiency;1～2 months of callus proper is fit to the transformation;the transformable efficiency of the whole light shine to co-cultivation is higher than that of 16?h and 8?h photoperiod and the whole dark;the proper negative pressure,when agrobacterium and callus cultivated,could increase transformable frequency and survival rate of callus.
出处
《南京林业大学学报（自然科学版）》
CAS
CSCD
北大核心
2004年第5期37-40,共4页
Journal of Nanjing Forestry University：Natural Sciences Edition
基金
国家自然科学基金资助项目(30370147)
关键词
农杆菌介导法
转化频率
存活率
辐照剂量
光照条件
负压处理
结缕草
Agrobacterium-mediated transformation
Transformable efficiency
Survival rate
Irradiation-dosage
Illumination condition
Negative pressure
Zoysia japonica
