摘要
以蛋白质为模板进行分子印迹得到的纳米“孔穴”结构生物材料,可以作为抗体、酶或其他天然生物结构的替代物及细胞支架材料,在生物技术和医学领域显示出广阔的应用前景。本文简要介绍了模板印迹的基本原理,分析了蛋白质模板印迹的特点,详细介绍了蛋白质包埋法、微球表面印迹法、平板表面印迹法和抗原表位法等蛋白质模板印迹方法,并对各种印迹方法进行了简要评述。
Nanocavity biomaterials with recognition specificity imprinted by using proteins as templates may successful serve as substitutes for antibodies,enzymes,and other native biological structures as well as cell bracket materials.It has numerous applications in biotechnology,medicine and so on.In this paper,the principle of template imprinting is introduced briefly,the specialty of template imprinting of proteins is analyzed,and the methods of template imprinting of proteins including protein entrapment,microbead surface imprinting,flat surface imprinting as well as the epitope are reviewed in details.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
2003年第5期640-644,共5页
Acta Academiae Medicinae Sinicae
基金
教育部优秀青年教师基金(2002123)
国家自然科学基金(29906008)资助~~
