摘要
从土壤中筛选得到一株产广谱、高活性抗真菌物质的链霉菌 2 4 # ,经测定对 2 5种植物病原真菌有显著拮抗作用 ,其次生代谢产物对病菌菌丝有断裂、扭曲、缢缩等致畸效应。 16SrDNA序列分析显示本菌株与模式菌株链霉菌DSM4 4 2 93T的 16SrDNA同源性为 98 93% ,但菌株形态特征、培养特征、细胞壁化学组分、生理生化特性等均不同于模式菌株 ,且DNA杂交率只有 2 2 5 4 % ,建议为链霉菌属的一个新种 ,命名为山东链霉菌 (Streptomycesshandon gensissp .nov .)。
The 24# strain of Streptomyces sp. producing broad-scope,high activities and antifungal substances were isolated from the soil. In screening process of agriculture antagonistic strains, streptomyces strain 24# was isolated from soil in Shandong. The antifungal substances have notable antagonistic effects of phytopathogens and can cause the mycelia of the phytopathogens breaking, twisting and constricting. The 16S rDNA sequence homology between the strain 24# and model strain S. DSM44293^(T) is 98.93%. However, the morphological and culture characteristics, the chemical components of the cell walls, the physiological and biochemical characteristics of the strain are different to those of the model strain, and the ratio of the DNA-DNA hybridization is only 22.54%. The name Streptomyces shandongensis sp. nov. is proposed for strain 24#.
出处
《微生物学报》
CAS
CSCD
北大核心
2004年第5期580-583,共4页
Acta Microbiologica Sinica
关键词
山东链霉菌
抗真菌活性
DNA—DNA杂交
鉴定
16S
RDNA序列
Streptomyces shandongensis sp. nov., Antifungal activity, DNA-DNA hybridization, Identification,(16S rDNA) sequence.
