摘要
目的通过对抑郁模型大鼠的快眼动睡眠剥夺 ,观察大鼠自主活动及海马 5 羟色胺 (5 HT)含量的改变 ,建立抑郁状态动物的睡眠剥夺模型。方法将大鼠分为正常对照组、抑郁模型组、抑郁模型+睡眠剥夺组和抑郁模型 +大平台对照组。在建立慢性轻度不可预见性应激的抑郁模型后 ,采用小平台水环境法对大鼠进行 72h快眼动睡眠剥夺 ,以开场试验检测大鼠自主活动 ,用高效液相色谱 荧光检测法测定大鼠海马 5 HT含量。结果接受 2 1d慢性轻度不可预见性应激后 ,大鼠的自主活动显著减少 (92 .12±4.42vs 2 2 .2 7± 3 .5 1,P <0 .0 0 1) ,93 .9% (3 1/3 3 )的大鼠出现抑郁样行为。快眼动睡眠剥夺后 ,92 % (11/12 )的抑郁模型 +睡眠剥夺组大鼠抑郁样行为被逆转 ,其自主活动和海马 5 HT含量与正常对照组相比无显著性差异 (P >0 .0 5 ) ,与大平台对照组相比显著增高 (P <0 .0 5 )。结论快眼动睡眠逆转抑郁模型大鼠的抑郁样行为并增加其海马 5 HT含量 。
Objective By investigating the effects of rapid eye movement sleep deprivation (REMSD) on depressive-behaviors and serotonin in hippocampus in rats treated with chronic mild unpredicted stresses, to establish animal model for further study of anti-depressive mechanisms of sleep deprivation. Methods The rats were divided into 4 groups: normal control, depression model, depression model with sleep deprivation and depression model with tank control.Exposing rats to unpredicted mild stressors for 21 consecutive days found rat depression model. 72 hours REMSD to the rats in depression model was performed by flowerpot technique. Autonomic behavior was measured with Open Field test and concentration of serotonin in hippocampus was assessed by high performance liquid chromatography coupled with fluorescence detection. ResultsAfter 21 days stresses, the autonomic activity of the rats were significantly reduced (92.12±4.42 vs 22.27±3.51,P<0.001), 94%(31/33) of them showed depressive-behaviors. After 72 hours REMSD, 92%(11/12)of the rats in depression model with sleep deprivation group showed a reversion of depressive-behaviors, the autonomic activity and concentration of serotonin in hippocampus in this group increased significantly than those in the rats in tank control (P< 0.05 ), and were no significant when compared with the normal controls (P>0.05).ConclusionRats showed a depressive-behaviors and a decline in serotonin in the hippocampus after 21 days stresses, while REMSD could reverse these effects. The results suggested that sleep deprivation to rat in depression model could be applied in the study of anti-depressive mechanisms of sleep deprivation.
出处
《中国行为医学科学》
CAS
CSCD
2004年第4期381-383,共3页
Chinese Journal of Behavioral Medical Science
