摘要
建立高效液相色谱法测定山丹酮缓释片中牡荆苷和金丝桃苷的含量。色谱柱LichrospherC18(5μm ,2 50mm× 4 6mm) ,流动相 :甲醇 -水 -冰醋酸 (50∶50∶1 ) ;检测波长 :340nm ;牡荆苷和金丝桃苷的线性范围分别为 :0 73～ 3 66μg (r =0 9999)和 0 63～ 3 1 6μg (r =0 9998) ;平均回收率分别为 :1 0 0 0 3% ,RSD =1 1 7% (n=5)和 99 81 % ,RSD =0 36 % (n =5)。本法操作简单 ,快速 ,准确 。
A HPLC method for the determination of Vitexin and Hyperoside in Shandantong sustained-release tablets was established. A Lichrospher C 18 column (4 6mm×250mm, 5μm) was used with methanol-water-glacial acetic acid (50∶50∶1) as mobile phase. The content was detected at a wavelength of 2340 nm. The linear range for Vitexin and Hyperoside were 0 73～3 66μg (r=0 9999) and 0 63～3 16μg (r=0 9998) respectively. The average recovery was 100 03%, RSD=1 17% (n=5) for Vitexin, and 99 81%, RSD=0 36% (n=5) for Hyperoside. The method was simple, rapid, accurate and feasible.
出处
《中国药事》
CAS
2004年第7期430-431,441,共3页
Chinese Pharmaceutical Affairs
