摘要
目的观察骨髓间质干细胞(MSC)移植治疗Duchenne肌营养不良症(DMD)动物模型dko小鼠后肌组织dystrophin/utrophin的表达情况。方法用体外培养纯化的第5代MSC经尾静脉移植治疗dko小鼠,移植后5、10、15、20周分别取实验鼠腓肠肌组织做荧光免疫组化检测dystrophin/utrophin的表达,计算阳性纤维的平均光密度。结果传3代以上呈集落生长的MSC均一性好,静脉移植免疫反应低。移植后5～20周dko小鼠肌膜组织dystrophin/utrophin免疫荧光表达强度有随着时间逐渐递增的趋势,移植15周前后肌膜荧光带光密度有显著性差异(P=0.035)。结论MSC在体内外均具有强大的可塑性,通过血液循环MSC可趋向病变肌组织,并分化为表达dystrophin/utrophin的肌纤维,干细胞移植对dko小鼠肌萎缩组织有一定修复作用。
Objective To observe dystrophin and utrophin expression in muscle tissues of Duchenne muscular dystrophy(DMD)mouse model(dko mouse)after having been treated with bone marrow mesenchymal stem cells(MSC)transplantation. Methods The fifth generation of MSCs, cultured in vitro, was tran-splanted into dko mice by tail vein. The fluorescent expression of dystrophin and utrophin in gastrocn-emius muscle tissue of dko mouse was detected and the average optical density of positive fibers was calculated. Results MSCs that had been cultured for three generations had good homogeneousness and the immunological reaction after vein transplantation was low. There was an increasing tendency of dystrophin and utrophin fluorescent expression in sarcolemma of dko mouse within 5-20 weeks. Signi-ficant difference existed in fluorescent average optical density of positive fibers fifteen weeks before and after cell transplantation. Conclusions MSC has strong plasticity both in vitro and in vivo. MSC has a trend to reach the injured muscle tissues and turn into muscle fibers, which express dystrophin and utrophin. There is some plerosis function for myatrophy of dko mouse by MSC transplantation.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
2004年第3期294-297,i004,共5页
Acta Academiae Medicinae Sinicae
基金
国家自然科学基金(30270732
30000057
30170337)
广东省科技攻关项目(2KM05501S)
广州市科委基金(2002J1-C0182)~~
