摘要
采用分割切块法测定了 3个草菇菌株 (V0 1、V0 2和V0 3)在低温下的致死时间。结果表明 :V0 1、V0 2和V0 3菌株在 0、 2和 4℃下的致死时间分别为 2 4、 4 8和 32h ,4 8、 88和 6 4h ,96、 192和 14 4h。用 0 0 1%吖啶橙诱变草菇菌株V0 1担孢子 30min ,致死率达 70 4 % ;对诱变处理的担孢子萌发形成的菌体进行低温临界致死处理 ,杀死低温敏感菌株 ,采用覆层平板法筛选出
The critical cryolethal time of three Volvariella volvacea strains V01,V02,V03 under 0 ℃,2 ℃ and 4 ℃ were found to be 24 h,48 h,32 h;48 h,88 h,64 h;as well as 96 h,192 h,144 h,respectively.The basidiospores of V01 were treated with 0 01% acridine orange at 25 ℃ for 30 min and the killing ratio reached to 70 4%.After spore germinating on IPDA plates,the colonies were treated for the critical cryolethal time to kill the sensitive ones.With cladding plate method,four low temperature resistent strains were selected out.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2004年第3期58-61,共4页
Journal of Nanjing Agricultural University
