摘要
目的 观察半年恢复期严重急性呼吸综合征 (SARS)患者血清中 SARS抗体对 SARS病毒介导细胞病变的抑制作用。方法 用酶联免疫吸附试验 (EL ISA)测定 12例 SARS恢复期患者血清中 SARS抗体 (Ig G)的吸光度 (A)值 ;用维持液按 1∶ 8稀释恢复期患者的血清 ,并与分离、培养和鉴定后不同稀释度的SARS病毒 (SARS Co )上清作用 ,再加入 Vero E6细胞悬液进行培养 ,观察 Vero E6细胞病变的程度。结果 12例恢复期患者半年前的 SARS抗体 A值范围为 0 .79～ 2 .0 1,半年后的 A值范围为 0 .81～ 2 .0 6。其加入 12例恢复期血清的 SARS Co Vero E6细胞病变程度至少降低 2 5 %以上。结论 SARS患者半年后恢复期血清的 SARS抗体滴度有上升趋势 ,它还可抑制 SARS病毒介导的细胞病变 ,对 SARS病毒有中和作用 ,推测
Objective To investigate inhibitory effect of serum severe acute respiratory syndrome(SARS)specific antibodies from convalescent patients after half an year of onset on SARSCoⅤmediated cytopathic response . Methods SARSCoⅤ immunoglobulin G(IgG) antibody was determinated by enzyme linked immunoadsorbent assay(ELISA). Twelve serum samples from convalescent patients, diluted by 1∶8 with maintenance medium, were mixed with the three dilution supernatants of SARSCoⅤ. SARSCoⅤ were isolated , cultured and identified by the Guangzhou Institute of Respiratory Disease,and cultured with Vero E6 cell suspension. The extent of cytopathic response was observed. Results The absorbance ( A ) value of SARSCoⅤ IgG antibody ranged from 0 81 to 2 06 in patients after half an year of SARS onset, and form 0 79 to 2 01 in patients before half an year of SARS onset. The extent of cytopathic response was decreased by more than 25% in all 12 convalescent patients, as compared with control serum. Conclusion The A value of SARSCoⅤ IgG antibody in serum of convalescent patients tended to elevate in half an year after SARS onset. SARSCoⅤ IgG antibody could inhibit SARSCoⅤmediated cytopathic response, indicating it might be one of protective antibodies.
出处
《中国危重病急救医学》
CAS
CSCD
2004年第7期409-412,共4页
Chinese Critical Care Medicine
基金
广东省广州市科技计划项目 ( 2 0 0 3 64 718)
