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在哺乳动物细胞中用于表达干扰RNA的启动子 被引量：6

Promoters Used in the Expression of siRNA in Mammalian Cells
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摘要 RNA干扰是由双链RNA诱导的特异性转录后基因表达沉默。目前用于在哺乳动物细胞中表达干扰RNA的启动子分为RNA聚合酶Ⅲ类启动子(polⅢ)及RNA聚合酶Ⅱ类启动子(polⅡ)。不同的启动子及经过不同方法改造后的启动子能满足不同研究目的的需要,从而极大地拓展了RNA干扰的应用范围。 RNA interference ( RNAi) is a process in which double-stranded RNA induces a sequence-dependent degradation of a congnate mRNA. RNAi has become an important tool in the study of gene function. The promoters used in the expression of siRNA in mammalian cells include RNA polymeraseⅢ and polymerase Ⅱ promoters. Different promoters, including modified promoters, have been developed to satisfy different research needs.
作者 刘苏虎 张镁 张王刚
机构地区 西安交通大学第二医院血液科西安市 西安交通大学第二医院病理科西安市
出处 《医学分子生物学杂志》 CAS CSCD 2004年第3期175-177,共3页 Journal of Medical Molecular Biology
关键词 RNA干扰 启动子 哺乳动物细胞 RNA interference, promoter, mammalian cells
分类号 Q782 [生物学—分子生物学]
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参考文献15

  • 1Kawasaki H, Taira K. Short hairpin type of dsRNAs that are controlled by tRNA (Val) promoter significantly induce RNAi-mediated gene silencing in the cytoplasm of human cells. Nucleic Acids Res, 2003, 31 (2): 700-7.
  • 2Oshima K, Kawasaki H, Soda Y, et al. Maxizymes and small hairpin-type RNAs that are driven by a tRNA promoter specifically cleave a chimeric gene associated with leukemia in vitro and in vivo. Cancer Res, 2003,63(20) :6 809-14.
  • 3Xia XG, Zhou H, Ding H, et al. An enhanced U6 promoter for synthesis of short hairpin RNA. Nucleic Acids Res,2003, 31 (17): e100 (1-5).
  • 4Paul CP, Good PD, Li SX, et al. Localized Expression of Small RNA Inhibitors in Human Cells. Mor Ther, 2003, 7(2): 237-47.
  • 5Boden D, Pusch O, Lee F, et al. Promoter choice affects the potency of HIV-1 specific RNA interference. Nucleic Acids Res, 2003, 31 (17): 5 033-8.
  • 6Xia H, Mao Q, Paulson HL, et al. siRNA-mediated gene silencing in vitro and in vivo. Nature Biotechnol, 2002,20:1006-10.
  • 7Tuschl T. Expanding small RNA interference. Nature biotech, 2002, 20: 446-8.
  • 8Paul CP, Good PD, Winer I, et al. Effective expression of small interfering RNA in human cells. Nature Biotechnol,2002, 20: 505-8.
  • 9Paddison PJ, Caudy AA, Bernstein E, et al. Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells. Genes & Dev, 2002, 16: 948-58.
  • 10Tran N, Cairns MJ, Dawes IW, et al. Expressing functional siRNAs in mammalian cells using convergent Transcfiption. BMC Biotechn, 2003, 3: 21-31.

同被引文献73

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二级引证文献11

医学分子生物学杂志
2004年 第3期

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