摘要
目的 :观察毛囊球部细胞种植到胶原 /壳聚糖多孔支架上皮肤重建情况。方法 :采用细胞培养技术和细胞支架种植技术 ,将培养的毛囊球部细胞种植到胶原 /壳聚糖多孔支架上 ,HE染色观察皮肤样结构形成情况 ,并作免疫组织化学鉴定 ,以证明细胞的来源属性。结果 :毛囊球部细胞种植到胶原 /壳聚糖多孔支架上可见皮肤样结构形成 ,表皮部分细胞分化良好 ,排列复层整齐 ,有角化现象 ,高分子与低分子角蛋白表达阳性。结论 :低传代培养的毛囊球部细胞在体外胶原
Objective: To observe the skin regeneration after hair follicle bulb cells were implanted into collagen/chitosan porous scaffolds in vitro. Methods: The cultured dorsal hair follicle bulb cells of 4d-old C57BL/6J mice were implanted into collagen/chitosan porous scaffolds in vitro. The skin regeneration was observed. Results: The skin-like structure was formed on the collagen/chitosan porous scaffolds where were cultured the hair follicle bulb cells before 4th passages. Conclusion: The skin-like structure is generated in vitro when early passages of cultured hair bulb cells are implanted into collagen/chitosan porous scaffolds.
出处
《浙江大学学报（医学版）》
CAS
CSCD
2004年第4期281-286,共6页
Journal of Zhejiang University(Medical Sciences)
基金
浙江科技厅科技计划重点项目 (2 0 0 3C2 30 12 )
浙江省卫生厅科研基金 (2 0 0 2 ZX0 34)
