摘要
目的 研究阿霉素磁性白蛋白纳米微粒对兔VX2肝肿瘤的介入治疗作用。方法 将成功接种VX2肝脏肿瘤的新西兰大白兔随机分成 4组 ,每组 6只 :A组为生理盐水对照组 ,肝动脉注射生理盐水 2 0mL ;B组为游离阿霉素组 ,肝动脉注入阿霉素 ( 1mg/kg) ;C组为阿霉素磁性白蛋白纳米微粒组 ,肝动脉注入阿霉素磁性白蛋白纳米微粒 32 .8mg/kg(含阿霉素 1mg) ;D组为阿霉素磁性白蛋白纳米微粒并在瘤区外加磁场组 ,肝动脉注入阿霉素磁性白蛋白纳米微粒 32 .8mg/kg(含阿霉素 1mg)。 4组实验动物于介入术后 7、14d行SCT肝脏扫描、术后 2 1d行胸腹联合扫描 ,测量肿瘤大小 ,检查肺部转移灶 ;各组实验动物均记录生存天数 ,生存满 90d则处死 ,全部实验动物均取肿瘤、肝和肺作组织病理学检查。结果 术后 7、14、2 1dA组平均肿瘤体积分别为( 4 .84± 0 .84 )cm3 、( 12 .6 7± 2 .18)cm3 和 ( 6 1.4 0± 7.87)cm3 ;B组为 ( 1.88± 0 .17)cm3 、( 7.0 8± 1.5 2 )cm3 和( 2 0 .0 7± 4 .4 8)cm3 ;C组为 ( 1.4 3± 0 .13)cm3 、( 3.6 9± 0 .77)cm3 和 ( 9.5 1± 2 .0 9)cm3 ;D组为 ( 1.18± 0 .2 6 )cm3 、( 1.94± 0 .4 7)cm3 和 ( 4 .2 3± 0 .92 )cm3 。B、C、D 3组肿瘤体积均小于同期对照组 。
Purpose: To study the inhibitory effects of adriamycin magnetic albumin nanoparticles (ADM-MANs) on liver VX2 tumor in rabbits by means of transcatheter hepatic arterial infusion. Methods: 24 rabbits with implantation of liver VX2 tumors, were randomly divided into 4 groups with different treatment infused via hepatic artery. Group A (control group), 20mL nomal saline; Group B (ADM group), free adriamycin (1 mg/kg); Group C (ADM-MANs group), ADM-MANs (32.8 mg/kg) containing 1 mg ADM; Group D (ADM-MANs + M group), ADM-MANs (32.8 mg/kg) containing 1 mg ADM with an external magnet stabilized on the tumor region. Spiral CT scans were performed to measure tumor volume 7 days, 14 days and 21 days after treatment respectively and thorax CT scans were performed 21 days after treatment to see if lung metastasis occurred. Survival durations of the animals were recorded and animals survived 90 days after treatment were sacrificed. Tumor tissues, liver tissues close to tumor and lung tissues were obtained and examined histologically. Results: The average tumor volumes 7 days, 14 days and 21 days after treatment were (4.84 ± 0.84) cm3, (12.67 ± 2.18) cm3 and (61.40 ± 7.87) cm3 in group A, (1.88 ± 0.17) cm3, (7.08 ± 1.52) cm 3 and (20.07 ± 4.48) cm3 in group B, (1.43 ± 0.13) cm3, (3.69 ± 0.77) cm3 and (9.51 ± 2.09) cm3 in group C, (1.18 ± 0.26) cm3, (1.94 ± 0.47) cm3 and (4.23 ± 0.92) cm3 in group D respectively. The average tumor volumes of group B, C and D were significantly smaller than that of group A the same time after treatment. Lung metastasis rate of group A (100%) was higher than any other groups (group B: 50%, group C: 33.3%, group D: 16.7%). The life span of group C was longer than group A or group B, while life span of group D was significantly longer than group A (P = 0.001), B (P = 0.001) or C (P = 0.002). Pathological results showed there were obvious intratumoral necrosis in group C and D. Tumor in group D exibit thoroughgoing necrosis, while liver tissue close to tumor incurred less damage. Conclusions: The results of this investigation showed that ADM-MANs treating liver tumor via hepatic artery infusion combined with external magnet was safe and feasible. ADM-MANs + M had significant inhibitory effect on liver VX2 tumor growth in rabbits with minor liver toxicity.
出处
《复旦学报（医学版）》
EI
CAS
CSCD
北大核心
2004年第4期383-386,i001,共5页
Hehai University Journal of Medical Sciences
关键词
阿霉素
磁性白蛋白纳米微粒
介入治疗
VX2肝肿瘤
Blood vessels
Computerized tomography
Histology
Living systems studies
Magnets
Nanostructured materials
Pathology
Proteins
Pulmonary diseases
Tissue
