摘要
本文研究了Se(Ⅳ)-SCN-RhB-吐温20高灵敏显色反应体系。Se(Ⅳ)与SCN^-和RhB^+形成三元离子缔合物,并吸附了染料琉氰酸盐,表观摩尔吸光系数2.0×10~6L·mol^(-1).cm^(-1),是碱性染料类分光光度法中最灵敏的。Se含量在0～2μg/25ml范围内遵从比尔定律。利用巯基棉分离干扰离子,选择性高。测定了食品中总硒,结果满意。
A highly sensitive spectrophotometric method for the determination of Se (Ⅳ) was developed by using Se(Ⅳ)-SCN -RhB--Tween 20 system. At pH 3. 9, a ternary complex of Se, thiocyanate and rho-damine B formed with the complexes of RhB-SCN being absorbed. The molar absorptivity was 2. 0×106L·mol-1·cm-1 and was the most sensitive one compared with other basic dye spectrophotometric method. Beer's law was obeyed in the concentration range of 0-80 ng/ml and the detection limit was 5 ng/ml. Interfering ions were eliminated by using thiohydroxy cotton, so that the selectivity of this method was good. Finally,Se in some foods was determined with good results.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1993年第9期1003-1007,共5页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金
关键词
硒
罗丹明B
食品
分光光度法
Selenium , Rhodamine B, Sensitivity,Food.
