摘要
目的 利用芯片技术探讨壬基酚 (nonylphend ,NP)对F1子代脑内分泌调控相关基因表达影响。方法 NP染毒F0亲代母鼠 ,分别提取NP组和对照组F1子代雄性 2日龄大鼠的脑组织mR NA ,分别用cy5和cy3逆转录标记 ,与Mouse4 0S基因表达谱芯片杂交 ,并对cy3、cy5荧光信号做扫描分析。结果 芯片结果显示实验组已知功能差异表达基因有 16条 ,其中下调基因有 13条 ,4条与内分泌调控密切相关。结论 NP可通过多种途径影响机体类固醇激素的合成、代谢及干扰雄性的生殖功能。
Objective To explore the effects of nonylphend on brain endocrine regulation-associated gene expression profiles of F1 generation rats by means of microarry technique. Methods The mRNA were extracted respectively from the brain of 2-day-old F1 generration rats whose F0 male generation were tested with and without nonylphend, then reversely transcribed to cDNA and labeled with cy5 and cy3 fluorescence. Subsequently, the cDNA probes were hybridized to the Mouse40S cDNA microarray and the fluorescent signals of cy5 and cy3 were scaned and analyzed. Results The result showed that had 16 identified genes expressed differently, including 13 down-regulated in which 4 were related to endocrine regulation. Conclusions Nonylphend can effect the synthesis and metabolism of Steroid Hormone, and disturb the reproductive function of the male rats.
出处
《预防医学情报杂志》
CAS
2004年第3期229-231,共3页
Journal of Preventive Medicine Information
基金
国家自然科学基金重点课题 ( 3 0 0 3 0 12 0 )
