摘要
以小叶锦鸡儿(Caragana microphylla)为研究对象,利用ISSR分子标记技术,对不同海拔梯度下小叶锦鸡儿6个居群遗传多样性进行分析。研究发现,8条ISSR扩增引物得到142条清晰的DNA条带,其中多态性条带为126条,多态位点百分率为88.73%。用POPGENE软件计算不同海拔梯度下各居群间和居群内的遗传参数,结果表明,Nei’s基因多样性为0.292 9,香农信息指数为0.441 7,表明在物种水平上小叶锦鸡儿居群具有较高的遗传多样性;低海拔区域居群的所有遗传多样性高于高海拔区域居群,表明海拔高度增加阻碍了居群间的基因交流,导致小叶锦鸡儿居群遗传多样性水平降低。
Caragana microphylla is the most dominant and constructive shrub species.We evaluated the level of genetic variation within and among populations sampled from two different altitude gradients using inter-simple sequence repeat polymorphism(ISSR) molecular markers.The results showed that eight ISSR primers generated 142 bands,of which 126(88.73%) were polymorphic.Based on Nei's Gst value calculated by POPGENE,the gene diversity(h) and Shannon's information index(I) was 0.292 9 and 0.441 7,respectively indicating considerable genetic variations at the species level.At the altitude gradients,the genetic diversity index in populations from low altitude region was higher than from high altitude region,shows that the gene exchange between populations was blocked by the increase of altitude.
出处
《草业科学》
CAS
CSCD
北大核心
2015年第4期552-559,共8页
Pratacultural Science
基金
国家自然科学基金项目(41201561)
中科院寒旱所人才基金项目(Y351151001)
甘肃省自然科学基金(145RJYA269)
