摘要
目的：通过正交试验优化胡黄连苷Ⅱ干预大鼠脑缺血模型的治疗剂量和时间窗。方法应用双侧颈总动脉结扎法建立大鼠前脑缺血模型，按照正交试验设计分组，经腹腔注射胡黄连苷Ⅱ干预治疗，酶联免疫吸附法法检测脑组织水通道蛋白4（AQP4）、基质金属蛋白酶9（MMP9）和环氧合酶2（COX2）的含量。结果胡黄连苷Ⅱ干预大鼠脑缺血模型的最佳治疗时间窗和剂量分别为缺血后1．5 h腹腔注射20 mg/kg对于AQP4、缺血后2．0 h和20 mg/kg对于MMP9，缺血后1．5 h和20 mg/kg对于COX2。结论从用药剂量最小化和治疗时间窗最大化的原则综合评价，胡黄连苷Ⅱ干预大鼠脑缺血模型的最佳治疗时间窗和剂量为缺血后1．5～2．0 h腹腔注射20 mg/kg。
Objective To optimize the therapeutic dose and time window of Picroside Ⅱ by orthogonal tests in rat cerebral ischemia model . Methods The forebrain ischemia models were established by bilateral common carotid artery occlusion method .The successful models were grouped according to orthogonal experimental design and intervented by injecting Picroside Ⅱ intraperitonenally at different post-ischemia times with different dosages .The contents of aquaporins 4 ( AQP4 ) , matrix metalloproteinases 9 ( MMP9 ) and cyclooxygenase 2 ( COX2 ) in brain tissue were measured by enzyme linked immunosorbent assay . Results The maximal therapeutic time and doses of Picroside Ⅱin rat cerebral ischemia models were 1.5 h post-ischemia with 20 mg/kg for AQP4, 2.0 h post-ischemia with 20 mg/kg for MMP9, and 1.5 h post-ischemia with 20 mg/kg for COX2. Conclusion According to the principle of lowest therapeutic dose with maximum time window , for decreasing the levels of AQP4, MMP9, and COX2 by Picroside Ⅱinjection in the rat ischemia models , optimized therapeutic dose and time window is 20 mg/kg and 1.5~2.0 h post-ischemia.
出处
《神经病学与神经康复学杂志》
2013年第3期158-161,共4页
Journal of Neurology and Neurorehabilitation
基金
国家自然科学基金项目(编号:81041092和81274116)
关键词
胡黄连苷Ⅱ
治疗剂量
时间窗
脑缺血
大鼠
Picroside Ⅱ
therapeutic dose
time window
cerebral ischemia
rat
