摘要
目的研究ｃ－ｍｙｃ基因与睾丸肿瘤的关系。方法应用核酸分子杂交方法检测２７例睾丸肿瘤（精原细胞瘤２３例，胚胎性瘤２例，畸胎瘤和淋巴瘤各１例）组织中ｃ－ｍｙｃ基因的扩散，并用免疫组织化学的亲和素－生物素－过氧化物酶复合物（ＡＢＣ）法检测ｃ－ｍｙｃ蛋白表达水平。结果２７例睾丸肿瘤中ｃ－ｍｙｃ基因扩增２例（７．４％），与肿瘤病理分级、临床分期无关；ｃ－ｍｙｃ蛋白阳性表达１２例（４４．１％），而正常睾丸组织和肿瘤旁组织为阴性，且与分化程度和病期早晚有关。结论睾丸肿瘤中ｃ－ｍｙｃ基因扩增不显著，但ｃ－ｍｙｃ蛋白表达与肿瘤恶性程度有明显相关性，可能成为睾丸肿瘤预后的指标。
To investigate the relationship between c-myc gene to and testis cancer.METHODS Dot bolt hybridization was used to evaluate the amplication of c-myc gene in 27 testiscancers (seminoma 23 cases, embryonal tumor 2 cases, teratoma and lymphoma 1 case each), also overexpression of c-myc protein was achieved by tile immunohistochemical method (ABC).RESULTS C-myc positive reactions were observed in 12 out of 27 tumors, and expression of c-mycwas related to the grade and stage of testic cancer.CONCLUSIONS It is suggested that the c-myc gene amplication is uncommon in testis cancer. Theincrease of c-myc protein is associated with testis cancer,which may be used as a possible marker of prognosis in testls cancer.
出处
《中国癌症杂志》
CAS
CSCD
1996年第3期156-158,共3页
China Oncology
关键词
睾丸肿瘤
癌基因
表达
扩增
免疫组织化学
杂交
testis neoplasms
oncogenes
expression
amplification
immunohistochemistry
hybridization
