摘要
为了研究中药消溃灵对消化性溃疡愈合的影响及其对胃粘膜保护作用，采用乙酸注射至大鼠胃窦部浆膜下形成慢性胃溃疡模型，测定溃疡指数，并用免疫组织化学的方法，观察表皮生长因子受体在溃疡周围粘膜的表达并进行图像分析。结果表明雷尼替丁组和消溃灵组溃疡指数显著低于模型组（Ｐ＜０．０１）。模型组和雷尼替丁组表皮生长因子受体较对照组增加（积分光密度值和阳性细胞占总面积的百分比为Ｐ＜０．０１），消溃灵组比模型组和雷尼替丁组进一步增加（积分光密度值为Ｐ＜０．０５，阳性细胞占总面积的百分比为Ｐ＜０．０１）。本文提示，消溃灵对表皮生长因子受体的表达有一定的促进作用，这可能是消溃灵促进胃溃疡愈合和胃粘膜保护作用的机理之一。
This study is aimed to investigate the effect of Traditional Chinese Medicinal herbs Xiao Kui Ling prescription (XKL) in the healing of chronic gastric ulcer in rats and its protective effect of gatric mucosa. A rat ulcer model induced by injecting acetic acid into the subserosa of stomach was used. The expression of ecpidermal growth factor receptor (EGFR) around the ulcer was observed immunohistochemically, and the immunoreaction was analysed by image analyser. The results showed that the ulcer index of the group treated with XKL and Ranitidim was lower than that of the model group(P<0 01). Expression of EGFR of model group and group treated with Ranitidim increased as compared wiht the control group (the light density and percent of area of positive cell P<0 01), and EGFR of group treated with XKL even inceased as compared with the model group and group treated with Ranitidim (the light density P<0 05, the percent of the total area of positive cell P<0 01). These suggest that XKL can improve the expression of EGFR, it might be one of the mechanisms that accelerates the healing of gastric ulcer and enhances protective effect.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
1998年第4期48-52,共5页
Chinese Journal of Histochemistry and Cytochemistry
基金
国家中医药管理局科研基金
关键词
胃溃疡
表皮生长因子受体
免疫组织化学
消溃灵
gastric ulcer
epidermal growth factor receptor
immunohistochemistry
Xiao Kui Ling prescription
