摘要
在HCl介质中,吖啶红3B与痕量NO-2发生亚硝化反应,使得吖啶红3B在激发波长为527nm,发射波长为556nm条件下的荧光强度明显下降,其下降程度在一定范围内与NO-2的加入量呈线性关系,从而建立了荧光熄灭法测定痕量NO-2的新方法。方法的线性范围为(4～320)×10-9g/mL,检出限为1.98×10-9g/mL。方法已用于环境水样中痕量NO-2的测定,回收率在95%～105%。
A new fluorescence quenching method was developed to determine nitrite in water samples. The method was based on the reaction between nitrite and Acridine Red 3B in hydrochloric acid medium. The fluorescence intensity was measured with excitation and emission wavelengths of 527 and 556 nm, respectively. The linear range for the determination of NO^-_2 was (4～320)×10^(-9) g/mL. The detection limit was 1.98×10^(-9) (g/mL) NO_2^-. It was applied to determine trace nitrite in water samples. The recoveries were 95%～105%.
出处
《分析试验室》
CAS
CSCD
北大核心
2004年第6期54-56,共3页
Chinese Journal of Analysis Laboratory
