摘要
目的比较市场上销售的三种检测牛奶中酪蛋白ELISA试剂盒效果。方法以脱脂奶粉、酪蛋白做作阳性添加成分,对三个品牌的ELISA试剂盒从回收率、精密度、特异性等方面进行比较。结果 ELISA试剂盒可以对多种食品进行酪蛋白含量检测,回收率、精密度等各项检测性能指标均可满足相关检测低限要求。结论 ELISA检测试剂盒无需大型分析仪器、操作简便快捷、适用于大量样品快速初筛和各种基层实验室食品品质监控。
Objective To compare the detection effect of three kinds of casein ELISA kits on the market.Methods The recovery,precision and specificity of three brands of ELISA kits were compared by adding positive ingredients,such as skim milk powder and casein.Results ELISA kits could detect casein content in a variety of foods.The recovery,precision and other performance could meet the relevant detection limit requirements.Conclusion The method is easy to operate and does not require any complex equipment.It is applicable to rapid screening for a large number of samples and a variety of primary laboratory food quality monitoring.
出处
《食品安全质量检测学报》
CAS
2012年第4期290-294,共5页
Journal of Food Safety and Quality
基金
公益性行业科研专项:常见食品过敏原的定量检测方法研究(10-46-1)
