摘要
目的观察参芪扶正注射液对人鼻咽癌CNE-2细胞的生长抑制作用,为其临床应用提供理论依据。方法将不同浓度的参芪扶正注射液与CNE-2细胞作用24、48、72和96h,用MTT法检测参芪扶正注射液对CNE-2细胞的生长抑制率,流式细胞仪分析细胞周期的变化、检测细胞凋亡率,荧光显微镜观察细胞形态学变化。结果参芪扶正注射液对CNE-2细胞的生长抑制率随药物浓度和作用时间的增加而增大。流式细胞仪分析显示参芪扶正注射液将CNE-2细胞明显阻滞于G1期。参芪扶正注射液浓度为8.0g/L作用48h,CNE-2细胞的凋亡率为39.3%,荧光显微镜下观察有凋亡细胞存在。结论参芪扶正注射液能够抑制人鼻咽癌CNE-2细胞的增殖,能使CNE-2细胞阻滞于G1期,可诱导CNE-2细胞发生一定程度的凋亡。
【Objective】To study the inhibiting effects of Shenqifuzheng injection on human nasopharyngeal car-cinoma CNE-2 cells. 【Methods】CNE-2 cells were incubated with Shenqifuzheng injection at various concentra-tions and time,cell growth inhibition was assessed by MTT assay,cell-cycle kinetics and apoptosis were analyzed by fluorenscence microscopy and flow cytometry. 【Results】The growth of CNE-2 cells was significantly inhibited by Shenqifuzheng injection in dose-dependent and time-dependent fashion. Analysis of flow cytometry showed that Shenqifuzheng injection could cause CNE-2 cell cycle to change and block CNE-2 cells in G1 phase. The apoptosis rate of CNE-2 cells was 39.3% after incubation with 8 g/L Shenqifuzheng injection for 48 hours. The apoptotic cells were observed under the fluorescence microscope. 【Conclusion】Shenqifuzheng injection suppresses the growth of CNE-2 cells in vitro by causing cell-cycle arrest and cell apoptosis.
出处
《中国医学工程》
2008年第4期269-271,274,共4页
China Medical Engineering
关键词
参芪扶正注射液
鼻咽癌
凋亡
Shenqifuzheng injection
nasopharyngeal carcinoma
apoptosis
