摘要
目的:探讨大鼠脑缺血再灌注后MMP-9的表达及早期头针干预对其的影响。方法:将205只SD大鼠随机分为假手术组、模型组和头针组。采用线栓法复制右侧大脑中动脉缺血再灌注模型;应用干湿比重法测定各组大鼠脑水含量;应用免疫组化和Western blot技术检测各组大鼠缺血侧脑组织MMP-9的表达。结果:头针组大鼠在再灌注24h、48h、72h,脑组织水含量较模型组显著降低(P<0.05)。再灌注各时间点头针组大鼠缺血侧脑组织MMP-9表达较模型组显著减少(P<0.05)。结论:早期进行头针治疗能够显著减少脑缺血再灌注后缺血侧脑组织中MMP-9的表达,减轻其脑水肿程度。MMP-9下调可能是该疗法拮抗脑缺血再灌注损伤的分子机制之一。
Objective:To investigate the effects of scalp acupuncture on MMP-9 expression in the rats with cerebral ischemia reperfusion for exploring the interaction mechanisms.Methods:205 Male SD rats were divided randomly into three groups:sham group,model group,and scalp acupuncture group.Focal cerebral ischemia was induced by intraluminal middle cerebral artery occlusion and reperfusion(MCAO/R) method by using a monofilament thread in male SD rats.We observed the brain water content of each set with the method of dry-wet weight;detected the positive expression and protein content of MMP-9 by immunohistochemistry and western blot methods respectively in brain tissue of each set.Results:Compared with model group,the brain water content has been significantly reduced in acupuncturing scalp group in IR24h,IR48h,IR72h after reperfusion(P<0.05).At the same time phase point,compared with the model set,the pinprick set's expression of MMP-9 lower obviously and showed the significant difference(P<0.05).Conclusion:Scalp-acupuncture therapy ameliorates brain edema in rats after acute cerebral ischemia/reperfusion by possibly inhibiting MMP-9 expression.
出处
《中医药学报》
CAS
2011年第3期107-111,共5页
Acta Chinese Medicine and Pharmacology
基金
教育部春晖计划(编号:Z2008-1-15012)
黑龙江省科技攻关项目(编号:GC08C424)
黑龙江省中医管理局项目(编号:HZ2008-0408)
黑龙江中医药大学科研基金项目(编号:200904)
关键词
头针
脑梗死
缺血再灌注损伤
MMP-9
Scalp acupuncture
Cerebral infarction
Cerebral ischemia reperfusion injury
Matrix Metalloproteinase-9
