摘要
目的分析牙周源性牙周牙髓联合病变患牙根管内口腔常见可疑致病菌的检出情况。方法收集因牙周损害导致的牙周牙髓联合病变患牙30例(实验组),开髓揭顶冲洗后用纸尖采集根管内组织,抽提DNA;以5例因正畸拔除的健康前磨牙作为阴性对照组。采用二步PCR技术对可疑病原微生物进行检测,包括牙周可疑致病微生物福赛类杆菌(T.forsythensis)、牙龈卟啉单胞菌(P.gingivalis)、具核梭杆菌(F.nucleatum)、中间普氏菌(P.intermedia)、牙密螺旋体(T.denticola)和牙髓感染相关微生物牙髓卟啉单胞菌(P.endodontalis)。结果实验组患牙根管内组织样本中有13个样本存在目标微生物感染,总检出率为43.3%;其中检出T.forsythensis感染6例(20.0%),P.intermedia感染5例(17.0%),P.gingivalis感染4例(13.0%),P.endodontalis感染4例(13.0%),F.nucleatum感染1例(3.0%),所有样本中均未检出T.denticola。阴性对照组牙根管内组织样本中目标微生物检出率为零。结论牙周源性牙周牙髓联合病变以牙周可疑致病微生物感染为主。
Objective To investigate the prevalences of common suspected pathogens in root canals from combined periodontal-endodontic lesions of periodontal origin. Methods Thirty teeth with periodontal-endodontic lesions of periodontal origin were collected(experiment group),and DNA was extracted from tissues in root canals by paper point after opening endodontic access and rinsing.Five healthy premolars extracted for orthodontics were served as negative controls.Two-step PCR was employed to detect the suspected pathogens,including periodontal suspected pathogens(T.forsythensis,P.gingivalis,F.nucleatum,P.intermedia and T.denticola) and endodontic infection related microorganism(P.endodontalis).Results Target microorganism infection was detected in 13(43.3%) tissue samples in root canals in experiment group.There were 6(20.0%) samples with T.forsythensis infection,5(17.0%) with P.intermedia infection,4(13.0%) with P.gingivalis infection,4(13.0%) with P.endodontalis infection and 1(3.0%) with F.nucleatum infection,while T.denticola was not detected in experiment group.Target microorganisms were not detected in all tissue samples in root canals in control group. Conclusion Periodontal suspected pathogen infection contributes to the majority of combined periodontal-endodontic lesions of periodontal origin.
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2011年第4期447-450,共4页
Journal of Shanghai Jiao tong University:Medical Science
基金
云南省科技联合基金(2008CD057)
云南省教育厅科学研究基金(2010Y207)~~
关键词
牙髓牙周联合病变
微生物
感染
periodontal-endodontic lesions
microorganism
infection
