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不同脱水条件下蝴蝶兰类原球茎的失水程度与耐脱水性 被引量:4

Dehydration Degree and Dehydration Tolerance of Phalaenopsis PLB Dryed under Different Conditions
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摘要 为了解蝴蝶兰类原球茎(PLB)耐脱水性规律,本研究通过取一定数目、鲜重的PLB样品分别经不同相对湿度或时间脱水后进行相关测定,结果表明经相对湿度52%下脱水2d的PLB含水率13.31g/gdw(脱水重/鲜重比值40.62%)是质膜损伤和胁迫致死的临界含水率。虽然不同脱水条件使材料达到接近的含水率,但较高湿度脱水对PLB组织细胞伤害较小。相较于含水率指标,PLB脱水重/鲜重比值与相对电导率、成活率有良好的线性关系。进一步的研究表明PLB脱水处理前用水浸泡不能明显提高脱水后的PLB脱水重/鲜重比值,但能明显提高成活率。从实验结果我们可以得出,慢速脱水蝴蝶兰PLB可减轻脱水损伤,降低脱水损伤对PLB起始含水率的敏感性;采用PLB脱水重/鲜重比值替代含水率表示材料失水程度是可取的。 For understanding the dehydration tolerance rule of Phalaenopsis PLB (protocorm-like bodies), in this paper, we carried out some determination by adopting a certain number and fresh weight of PLB samples and dehydrating at different relative humidity and time, respectively. The results showed that the critical water content of plasma membrane damage and PLB death was 13.31 g/gdw, and its DhW/FW (dehydrated weight/fresh weight) was 40.62% under the codition of dehydration at 52% RH for 2 days. Although the w...
出处 《基因组学与应用生物学》 CAS CSCD 北大核心 2010年第5期931-936,共6页 Genomics and Applied Biology
基金 福建省自然科学基金资助项目(2009J01079)资助
关键词 蝴蝶兰类原球茎 脱水条件 失水程度 脱水损伤 Phalaenopsis PLB Dehydrate condition Dehydrate degree Dehydrate damage
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