摘要
目的:表达和纯化弓形虫SAG1,分析其免疫原性。方法:将诱导表达SAG1后菌体裂解,离心,分别收集其上清和沉淀;亲和层析分离纯化裂解上清液中的SAG1,SDS-PAGE和Western blot鉴定SAG1纯度和免疫原性。结果:诱导后重组体pGEX-SAG1/Bl21超声裂解上清液、8M脲溶解沉淀的上清中都含有融合蛋白GST-SAG1,从超声裂解上清液中纯化获得融合蛋白GST-SAG1。结论:亲和层析获得具有免疫原性的SAG1。
Objective To express and purification the major surface antigen 1 truncated fragment of Toxoplasma gondii and analyse its immunogenicity.Methods The recombinant clone pGEX-SAG1/Bl21 was induced with IPTG to express target protein SAG1,and expressed recombinant protein was purified by affinity chromatography;The purifity and immunogenicity of this protein was analyzed by SDS-PAGE and western-blot reacting with the sera from rabbits infecting with Toxoplasma gondii.Results the major surface antigen 1 both exi...
出处
《晓庄学院学报（医学版）》
2007年第1期23-25,共3页
Journal of Hunan Normal University(Medical Sciences)
基金
湖南省卫生厅科研基金资助项目(B2005109)
关键词
弓形虫
SAG1
纯化
免疫原性
Toxoplasma gondii
major surface antigen 1
purification
immunogenicity
