摘要
利用枯草芽孢杆菌WY-45所产甘露聚糖酶水解椰子壳甘露聚糖,产物以低聚甘露糖为主,得率为44%。所得糖液采用活性炭柱层析,分离纯化结晶,得到了S1-S4四种糖晶体,经鉴定为甘露二糖、甘露三糖、甘露四糖和半乳甘露四糖。
Copra mannan was hydrolyzed by the crude β-mannanase from Bacillus subtilis WY-45 to yield mannooligosaccharidesas the main products, and the yield was up to 44%. The hydrolyzate was separated by active carbon column chromatography,and four kinds of mannooligosaccharides were obtained as crystalline state. They were identified as manobiose, mannotriose,mannotetrose andg alactosylmannotetrose, respectively.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第z1期58-60,共3页
Food Science
关键词
低聚甘露糖
Β-甘露聚糖酶
枯草芽孢杆菌
制备
结晶
mannooligosaccharides
β-mannanase
Bacillus subtilis
preparation
crystallization
